LS 174T-CAS9 cell line

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LS 174T-CAS9 Order

LS 174T-CAS9 cell line
Catalog#
YC-C088-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
LS 174T-CAS9 cell line
Order

Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
LS 174T-CAS9
Cell Morphology
Epithelial-like,adherent
Passage ratio
1:2~1:4
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the LS 174T cell line, screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a LS 174T-CAS9 cell line that can maintain long-term, stable and high expression.
Cutting Efficiency Validation
LS 174T-CAS9 cell line Product Validation Data(RT-QPCR)
Note: The above figure shows the sequencing peaks of the LS-174T-CAS9 stable cell pool which is electroporated by NONO gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
Loci STR Info (Sample Cell)
Sample Cell Line:LS 174T
STR Info (Cell bank)
Cell Line:LS174T
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 1116111415.0
D13S317 10101010
D7S820 11111111
D16S539 11131113
VWA 151617.0151718.0
TH01 6767
AMEL XXXX
TPOX 8989
CSF1PO 10131014
D12S391 1620
FGA 202122.0
D2S1338 1822
D21S11 293031.0
D18S51 1113
D8S1179 1216
D3S1358 1517
D6S1043 121314.0
PENTAE 1516
D19S433 1415
PENTAD 1010
D1S1656 121318.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • Easy to use:Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO:These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell:Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03
LS 174T-CAS9 cell line
LS 174T-CAS9
Catalog#
YC-C088-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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